In this paper the possibilities that Matlab provides to design, implementation and monitoring programs of autonomous navigation for mobile robots, on both simulated and real platforms , through its new toolbox for robotics will be explored. Robotics System Toolbox has established itself as a solid tool of integration between Matlab and robot operating under ROS environment. It will be studied the tools available in this toolbox that allow achieving the connection between the two platforms, in addition to the generation of algorithms of location, path planning, mapping and autonomous navigation.